Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98295

v-SNARE Vti1p rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

v-SNARE Vti1p rabbit pAb from ELK Biotechnology Read more ›

v-SNARE Vti1p rabbit pAb
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v-SNARE Vti1p rabbit pAb

Product Description

v-SNARE Vti1p rabbit pAb is a research-use antibody from ELK Biotechnology for studying v-SNARE Vti1p in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.,similarity:Belongs to the VTI1 family.,subunit:Found in a complex with VAMP8 and STX7 in the liver. Forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Part of the SNARE core complex containing STX7, STX8 and VAMP8.,tissue specificity:Expressed in all tissues examined.,

Additional antibody information

Specificity v-SNARE Vti1p Polyclonal Antibody detects endogenous levels of v-SNARE Vti1p protein.
Validation evidence captions Western Blot analysis of RAT-HEART cells using v-SNARE Vti1p Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: v-SNARE Vti1p
Collaborator ELK Biotechnology
Partner model ES4484
Target v-SNARE Vti1p
Research areas>>SNARE interactions in vesicular transport
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from the Internal region of human v-SNARE Vti1p.
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in all tissues examined.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
v-SNARE Vti1p
Also known as
VTI1B; VTI1; VTI1L; VTI1L1; VTI2; Vesicle transport through interaction with t-SNAREs homolog 1B; Vesicle transport v-SNARE protein Vti1-like 1; Vti1-rp1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from the Internal region of human v-SNARE Vti1p.
Observed band
26kD
Cellular localization
Early endosome membrane ; Single-pass type IV membrane protein . Late endosome membrane ; Single-pass type IV membrane protein . Lysosome membrane . Cytoplasmic granule . Recycling endosome membrane ; Single-pass type IV membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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