NHE-6 rabbit pAb from ELK Biotechnology Read more ›
NHE-6 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NHE-6 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a sodium-hydrogen exchanger that is amember of the solute carrier family 9. The encoded protein localizes to early and recycling endosomes and may be involved in regulating endosomal pH and volume. Defects in this gene are associated with X-linked syndromic mental retardation, Christianson type. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010],
| Specificity | NHE-6 Polyclonal Antibody detects endogenous levels of NHE-6 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of RAW cells using NHE-6 Polyclonal Antibody | Western blot analysis of SLC9A6 Antibody. The lane on the right is blocked with the SLC9A6 peptide. | Western blot analysis of the lysates from COLO205 cells using SLC9A6 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4482 |
| Target | NHE-6 |
| Research areas | >>Cardiac muscle contraction |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SLC9A6. AA range:551-600 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous; but is most abundant in mitochondrion-rich tissues such as brain, skeletal muscle and heart. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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