PFAAP5 rabbit pAb from ELK Biotechnology Read more ›
PFAAP5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PFAAP5 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,
| Specificity | PFAAP5 Polyclonal Antibody detects endogenous levels of PFAAP5 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of LOVO cells using PFAAP5 Polyclonal Antibody | Western blot analysis of lysates from LOVO cells, using N4BP2L2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using N4BP2L2 antibody. | Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES4472 |
| Target | PFAAP5 |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human N4BP2L2. AA range:358-407 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Colon,Thalamus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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