Mindin rabbit pAb from ELK Biotechnology Read more ›
Mindin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Mindin in WB, IHC workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Cell adhesion protein that promote adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens.,similarity:Contains 1 spondin domain.,similarity:Contains 1 TSP type-1 domain.,tissue specificity:Expressed in normal lung tissues but not in lung carcinoma cell lines.,
| Specificity | Mindin Polyclonal Antibody detects endogenous levels of Mindin protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of COS-7 cells using Mindin Polyclonal Antibody | Western blot analysis of the lysates from HUVECcells using SPON2 antibody. | Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES4470 |
| Target | Mindin |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SPON2. AA range:71-120 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in normal lung tissue but not in lung carcinoma cell lines. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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