Epac rabbit pAb from ELK Biotechnology Read more ›
Epac rabbit pAb is a research-use antibody from ELK Biotechnology for studying Epac in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
caution:It is uncertain whether Met-1 or Met-43 is the initiator.,domain:The DEP domain is involved in membrane localization independent from regulation by cAMP.,function:Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP.,similarity:Contains 1 cyclic nucleotide-binding domain.,similarity:Contains 1 DEP domain.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,tissue specificity:Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.,
| Specificity | Epac Polyclonal Antibody detects endogenous levels of Epac protein. |
|---|---|
| Validation evidence captions | Western blot analysis of the lysates from HeLa cells using RAPGEF3 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4469 |
| Target | Epac |
| Research areas | >>Rap1 signaling pathway, >>cAMP signaling pathway, >>Phospholipase D signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>Leukocyte transendothelial migration, >>Long-term potentiation, >>Serotonergic synapse |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RAPGEF3. AA range:712-761 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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