PAK4/5/6 rabbit pAb from ELK Biotechnology Read more ›
PAK4/5/6 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PAK4/5/6 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | PAK4/5/6 Polyclonal Antibody detects endogenous levels of PAK4/5/6 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from K562 cells, treated with PMA 125ng/ml 30', using PAK4/5/6 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using PAK4/5/6 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4458 |
| Target | PAK4/5/6 |
| Research areas | >>ErbB signaling pathway, >>Ras signaling pathway, >>Axon guidance, >>Focal adhesion, >>T cell receptor signaling pathway, >>Regulation of actin cytoskeleton, >>Human immunodeficiency virus 1 infection, >>MicroRNAs in cancer, >>Renal cell carcinoma |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PAK4/5/6. AA range:441-490 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highest expression in prostate, testis and colon. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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