Troponin I-C rabbit pAb from ELK Biotechnology Read more ›
Troponin I-C rabbit pAb is a research-use antibody from ELK Biotechnology for studying Troponin I-C in WB, IHC, IF, ELISA workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
| Specificity | Troponin I-C Polyclonal Antibody detects endogenous levels of Troponin I-C protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of mouse cells using Troponin I-C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunofluorescence analysis of HepG2 cells, using TNNI3 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using TNNI3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from mouse heart cells, using TNNI3 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4400 |
| Target | Troponin I-C |
| Research areas | Antibody Research |
| Reactivity | Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from mouse TNNI3. AA range:5-54 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Mouse | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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