SR-5B rabbit pAb from ELK Biotechnology Read more ›
SR-5B rabbit pAb is a research-use antibody from ELK Biotechnology for studying SR-5B in WB, IF, ELISA workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins. Probably involved in anxiety and depression.
| Specificity | SR-5B Polyclonal Antibody detects endogenous levels of SR-5B protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of COLO cells using SR-5B Polyclonal Antibody | Immunofluorescence analysis of HUVEC cells, using HTR5B Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO cells, using HTR5B Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using HTR5B antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4399 |
| Target | SR-5B |
| Research areas | Antibody Research |
| Reactivity | Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from rat HTR5B. AA range:280-329 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Mouse | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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