Na+/K+-ATPase α1 (phospho Ser23) rabbit pAb from ELK Biotechnology Read more ›
Na+/K+-ATPase α1 (phospho Ser23) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Na+/K+-ATPase α1 in WB, IF, ELISA workflows with listed reactivity for Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The ATPase Na+/K+ transporting subunit alpha 1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
| Specificity | Phospho-Na+/K+-ATPase α1 (S23) Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein only when phosphorylated at S23. |
|---|---|
| Validation evidence captions | Western Blot analysis of rat cells using Phospho-Na+/K+-ATPase α1 (S23) Polyclonal Antibody | Immunofluorescence analysis of NIH/3T3 cells, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from rat brain, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4388 |
| Target | Na+/K+-ATPase α1 |
| Research areas | Antibody Research |
| Reactivity | Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IF: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from rat ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Ser23. AA range:15-64 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Rat | Western Blot: 1/500 - 1/2000. IF: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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