Na+/K+-ATPase α1 (phospho Ser23) rabbit pAb
Na+/K+-ATPase α1 (phospho Ser23) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Na+/K+-ATPase α1 (phospho Ser23) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Na+/K+-ATPase α1.
Background
The ATPase Na+/K+ transporting subunit alpha 1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Additional antibody information
| Specificity | Phospho-Na+/K+-ATPase α1 (S23) Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein only when phosphorylated at S23. |
|---|---|
| Validation evidence captions | Western Blot analysis of rat cells using Phospho-Na+/K+-ATPase α1 (S23) Polyclonal Antibody | Immunofluorescence analysis of NIH/3T3 cells, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from rat brain, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | Na+/K+-ATPase α1 |
| Also known as | ATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IF: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Rat |
| Observed band | 113kD |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IF: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from rat ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Ser23. AA range:15-64 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Rat | Western Blot: 1/500 - 1/2000. IF: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.