ABM98250
Bcl-2 (phospho Ser70) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Bcl-2 (phospho Ser70) rabbit pAb from ELK Biotechnology Read more ›
Related formats
Zoom
Product Description
Bcl-2 (phospho Ser70) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Bcl-2.
Background
BCL2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Additional antibody information
| Specificity | Phospho-Bcl-2 (S70) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at S70. |
|---|---|
| Validation evidence captions | Western Blot analysis of JK cells using Phospho-Bcl-2 (S70) Polyclonal Antibody | Western Blot analysis of Jurkat cells using Phospho-Bcl-2 (S70) Polyclonal Antibody | Western blot analysis of BCL2 (Phospho-Ser70) Antibody. The lane on the right is blocked with the BCL2 (Phospho-Ser70) peptide. |
Key Facts
Product type
Primary Antibody›
Target: Bcl-2
| Target | Bcl-2 |
| Also known as | BCL2; Apoptosis regulator Bcl-2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Mouse;Rat |
| Observed band | 26kD |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BCL2 around the phosphorylation site of Ser70. AA range:36-85 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Why researchers trust ABMIUM
2,000+
Researchers trust us
50,000+
Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Mouse | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Bcl-2
Also known as
BCL2; Apoptosis regulator Bcl-2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Mouse;Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human BCL2 around the phosphorylation site of Ser70. AA range:36-85
Observed band
26kD
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
Customer Reviews
Recently Viewed