Bcl-2 (phospho Ser70) rabbit pAb from ELK Biotechnology Read more ›
Bcl-2 (phospho Ser70) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Bcl-2 in WB, ELISA workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
BCL2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
| Specificity | Phospho-Bcl-2 (S70) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at S70. |
|---|---|
| Validation evidence captions | Western Blot analysis of JK cells using Phospho-Bcl-2 (S70) Polyclonal Antibody | Western Blot analysis of Jurkat cells using Phospho-Bcl-2 (S70) Polyclonal Antibody | Western blot analysis of BCL2 (Phospho-Ser70) Antibody. The lane on the right is blocked with the BCL2 (Phospho-Ser70) peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4387 |
| Target | Bcl-2 |
| Research areas | Antibody Research |
| Reactivity | Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BCL2 around the phosphorylation site of Ser70. AA range:36-85 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Mouse | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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