Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98222

CD155 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CD155 rabbit pAb from ELK Biotechnology Read more ›

CD155 rabbit pAb
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CD155 rabbit pAb

Product Description

CD155 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD155 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008],

Additional antibody information

Specificity CD155 Polyclonal Antibody detects endogenous levels of CD155 protein.
Validation evidence captions Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of KB cells using CD155 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of KB cells using CD155 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: CD155
Collaborator ELK Biotechnology
Partner model ES4358
Target CD155
Research areas>>Cell adhesion molecules
Reactivity Human,Mouse,Rat
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human PVR. AA range:81-130
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Liver,Pancreas,Placenta,Plasma,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CD155
Also known as
PVR; PVS; Poliovirus receptor; Nectin-like protein 5; NECL-5; CD155
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human PVR. AA range:81-130
Observed band
70kD
Cellular localization
Isoform Alpha: Cell membrane; Single-pass type I membrane protein.; Isoform Delta: Cell membrane; Single-pass type I membrane protein.; Isoform Beta: Secreted.; Isoform Gamma: Secreted.
Dilution range
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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