FAS rabbit pAb
FAS rabbit pAb from ELK Biotechnology Read more ›
Product Description
FAS rabbit pAb is an ELK Biotechnology antibody for research workflows involving FAS.
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, s
Additional antibody information
| Specificity | FAS Polyclonal Antibody detects endogenous levels of FAS protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HEB cells using FAS Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | FAS |
| Also known as | FAS; APT1; FAS1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 37kD |
| Cellular localization | Isoform 1: Cell membrane ; Single-pass type I membrane protein . Membrane raft .; Isoform 2: Secreted.; Isoform 3: Secreted.; Isoform 4: Secreted.; Isoform 5: Secreted.; Isoform 6: Secreted. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Platinum drug resistance;>>MAPK signaling pathway;>>Cytokine-cytokine receptor interaction;>>p53 signaling pathway;>>Apoptosis;>>Necroptosis;>>Natural killer cell mediated cytotoxicity;>>TNF signaling pathway;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Type I diabetes mellitus;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Chagas disease;>>African trypanosomiasis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Autoimmune thyroid disease;>>Allograft rejection;>>Graft-versus-host disease;>>Lipid and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human FAS. AA range:51-100 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.