HLA-DQB1/2 rabbit pAb from ELK Biotechnology Read more ›
HLA-DQB1/2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying HLA-DQB1/2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
major histocompatibility complex, class II, DQ beta 1(HLA-DQB1) Homo sapiens HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marro
| Specificity | HLA-DQB1/2 Polyclonal Antibody detects endogenous levels of HLA-DQB1/2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of PC12 cells using HLA-DQB1/2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4222 |
| Target | HLA-DQB1/2 |
| Research areas | >>Phagosome, >>Cell adhesion molecules, >>Antigen processing and presentation, >>Hematopoietic cell lineage, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>Intestinal immune network for IgA production, >>Type I diabetes mellitus, >>Leishmaniasis, >>Toxoplasmosis, >>Staphylococcus aureus infection, >>Tuberculosis, >>Influenza A, >>Human T-cell leukemia virus 1 infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Asthma, >>Autoimmune thyroid disease, >>Inflammatory bowel disease, >>Systemic lupus erythematosus, >>Rheumatoid arthritis, >>Allograft rejection, >>Graft-versus-host disease, >>Viral myocarditis |
| Reactivity | Human |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human HLA-DQB1/HLA-DQB2. AA range:131-180 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cell,Blood,Colon,Peripheral blood,Peripheral bloo |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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