Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98115

STING/TMEM173 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

STING/TMEM173 rabbit pAb from ELK Biotechnology Read more ›

STING/TMEM173 rabbit pAb
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STING/TMEM173 rabbit pAb

Product Description

STING/TMEM173 rabbit pAb is a research-use antibody from ELK Biotechnology for studying STING/TMEM173 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014],

Additional antibody information

Specificity TMEM173 Polyclonal Antibody detects endogenous levels of TMEM173 protein.
Validation evidence captions Western Blot analysis of HeLa, K562, 4T1 cells using TMEM173 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-Breast-cancer, antibody was diluted at 1:100

Key Facts

Product typePrimary Antibody Target: STING/TMEM173
Collaborator ELK Biotechnology
Partner model ES4218
Target STING/TMEM173
Research areas>>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>Shigellosis, >>Human cytomegalovirus infection, >>Herpes simplex virus 1 infection, >>Human immunodeficiency virus 1 infection, >>Coronavirus disease - COVID-19
Reactivity Human,Mouse
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Transmembrane protein 173 at AA range: 301-350
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
STING/TMEM173
Also known as
TMEM173; ERIS; MITA; STING; Transmembrane protein 173; Endoplasmic reticulum interferon stimulator; ERIS; Mediator of IRF3 activation; hMITA; Stimulator of interferon genes protein; hSTING
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIHCIFELISA
Immunogen
Synthesized peptide derived from Transmembrane protein 173 at AA range: 301-350
Observed band
38kD
Cellular localization
Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein . Mitochondrion outer membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . In response to double-stranded DNA stimulation, translocates from the endoplasmic reticulum through the endoplasmic reticulum-Golgi intermediate compartment and Golgi to post-Golgi vesicles, where the kinase TBK1 is recruited (PubMed:19433799, PubMed:30842659, PubMed:30842653, PubMed:29694889). Upon cGAMP-binding, translocates to the endoplasmic reticulum-Golgi interme
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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