Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98112

HRP-3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

HRP-3 rabbit pAb from ELK Biotechnology Read more ›

HRP-3 rabbit pAb
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HRP-3 rabbit pAb

Product Description

HRP-3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying HRP-3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Enhances DNA synthesis and may play a role in cell proliferation.,similarity:Belongs to the HDGF family.,similarity:Contains 1 PWWP domain.,tissue specificity:Detected in testis, heart, spinal cord and brain.,

Additional antibody information

Specificity HRP-3 Polyclonal Antibody detects endogenous levels of HRP-3 protein.
Validation evidence captions Western Blot analysis of NIH-3T3, PC12 cells using HRP-3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-Lymph-nodes, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-Lymph-nodes, antibody was diluted at 1:100

Key Facts

Product typePrimary Antibody Target: HRP-3
Collaborator ELK Biotechnology
Partner model ES4214
Target HRP-3
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
ConjugationHRP
Dilution rangeWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human HDGFRP3. AA range:131-180
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Detected in testis, heart, spinal cord and brain.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
HRP-3
Also known as
HDGFRP3; HDGF2; CGI-142; Hepatoma-derived growth factor-related protein 3; HRP-3; Hepatoma-derived growth factor 2; HDGF-2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
HRP
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human HDGFRP3. AA range:131-180
Observed band
23kD
Cellular localization
Nucleus .
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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