FOXP3 rabbit pAb from ELK Biotechnology Read more ›
FOXP3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying FOXP3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Pig. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
| Specificity | FOXP3 Polyclonal Antibody detects endogenous levels of FOXP3 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of PC12 cells using FOXP3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-tonsilla, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-tonsilla, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4176 |
| Target | FOXP3 |
| Research areas | >>Th17 cell differentiation, >>Inflammatory bowel disease |
| Reactivity | Human,Mouse,Rat,Pig |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human FOXP3. AA range:381-430 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Pig |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Pig | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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