IL-12A p35 rabbit pAb from ELK Biotechnology Read more ›
IL-12A p35 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL-12A in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008],
| Specificity | IL-12A p35 Polyclonal Antibody detects endogenous levels of IL-12A p35 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of MCF7 cells using IL-12A p35 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4172 |
| Target | IL-12A |
| Research areas | >>Cytokine-cytokine receptor interaction, >>Toll-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Alcoholic liver disease, >>Type I diabetes mellitus, >>Pertussis, >>Legionellosis, >>Leishmaniasis, >>Chagas disease, >>African trypanosomiasis, >>Malaria, >>Toxoplasmosis, >>Amoebiasis, >>Tuberculosis, >>Measles, >>Influenza A, >>Herpes simplex virus 1 infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Inflammatory bowel disease, >>Allograft rejection, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human IL12A. AA range:31-80 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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