Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98060

DRA rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

DRA rabbit pAb from ELK Biotechnology Read more ›

DRA rabbit pAb
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DRA rabbit pAb

Product Description

DRA rabbit pAb is a research-use antibody from ELK Biotechnology for studying DRA in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a transmembrane glycoprotein that transports chloride ions across the cell membrane in exchange for bicarbonate ions. It is localized to the mucosa of the lower intestinal tract, particularly to the apical membrane of columnar epithelium and some goblet cells. The protein is essential for intestinal chloride absorption, and mutations in this gene have been associated with congenital chloride diarrhea. [provided by RefSeq, Oct 2008],

Additional antibody information

Specificity DRA Polyclonal Antibody detects endogenous levels of DRA protein.
Validation evidence captions Western Blot analysis of 293, HeLa cells using DRA Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: DRA
Collaborator ELK Biotechnology
Partner model ES4147
Target DRA
Research areas>>Pancreatic secretion, >>Mineral absorption
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from the C-terminal region of human DRA.
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Colon,Lung,Rectum tumor,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
DRA
Also known as
SLC26A3; DRA; Chloride anion exchanger; Down-regulated in adenoma; Protein DRA; Solute carrier family 26 member 3
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from the C-terminal region of human DRA.
Observed band
84kD
Cellular localization
Apical cell membrane ; Multi-pass membrane protein . Membrane ; Multi-pass membrane protein . Localized in sperm membranes. Midpiece of sperm tail. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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