Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98051

Inhibin β-B rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Inhibin β-B rabbit pAb from ELK Biotechnology Read more ›

Inhibin β-B rabbit pAb
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Inhibin β-B rabbit pAb

Product Description

Inhibin β-B rabbit pAb is a research-use antibody from ELK Biotechnology for studying Inhibin β-B in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate a subunit of the dimeric activin and inhibin protein complexes. These complexes activate and inhibit, respectively, follicle stimulating hormone secretion from the pituitary gland. Polymorphisms near this gene are associated with pre-eclampsia in female human patients. [provided by RefSeq, Aug 2016],

Additional antibody information

Specificity Inhibin β-B Polyclonal Antibody detects endogenous levels of Inhibin β-B protein.
Validation evidence captions Western Blot analysis of HeLa cells using Inhibin β-B Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of YT5408wb4147442094 lysis using INHBB Antibody antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100

Key Facts

Product typePrimary Antibody Target: Inhibin β-B
Collaborator ELK Biotechnology
Partner model ES4138
Target Inhibin β-B
Research areas>>Cytokine-cytokine receptor interaction, >>TGF-beta signaling pathway, >>Signaling pathways regulating pluripotency of stem cells
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Inhibin beta B chain at AA range: 351-400
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Inhibin β-B
Also known as
INHBB; Inhibin beta B chain; Activin beta-B chain
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCIFELISA
Immunogen
Synthesized peptide derived from Inhibin beta B chain at AA range: 351-400
Observed band
45kD
Cellular localization
Secreted.
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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