Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98033

Glut5 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Glut5 rabbit pAb from ELK Biotechnology Read more ›

Glut5 rabbit pAb
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Glut5 rabbit pAb

Product Description

Glut5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Glut5 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a fructose transporter responsible for fructose uptake by the small intestine. The encoded protein also is necessary for the increase in blood pressure due to high dietary fructose consumption. [provided by RefSeq, Jun 2016],

Additional antibody information

Specificity Glut5 Polyclonal Antibody detects endogenous levels of Glut5 protein.
Validation evidence captions Western Blot analysis of K562 cells using Glut5 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from K562 cells, using SLC2A5 Antibody.

Key Facts

Product typePrimary Antibody Target: Glut5
Collaborator ELK Biotechnology
Partner model ES4118
Target Glut5
Research areas>>Carbohydrate digestion and absorption
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the N-terminal region of human SLC2A5. AA range:31-80
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level) (PubMed:7619085). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Glut5
Also known as
SLC2A5; GLUT5; Solute carrier family 2, facilitated glucose transporter member 5; Fructose transporter; Glucose transporter type 5, small intestine; GLUT-5
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human SLC2A5. AA range:31-80
Observed band
55kD
Cellular localization
Apical cell membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cell membrane, sarcolemma . Localized on the apical membrane of jejunum villi, but also on lateral plasma membranes of the villi. Transport to the cell membrane is dependent on RAB11A. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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