ABM98021
ALPP/ALPPL2 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ALPP/ALPPL2 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ALPP/ALPPL2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving PLAP.
Background
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015],
Additional antibody information
| Specificity | ALPP/ALPPL2 Polyclonal Antibody detects endogenous levels of ALPP/ALPPL2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293 cells using ALPP/ALPPL2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of customer's sample using ALPP/ALPPL2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from 293 cells, using ALPP/ALPPL2 Antibody. |
Key Facts
Product type
Primary Antibody›
Target: PLAP
| Target | PLAP |
| Also known as | ALPP; PLAP; Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; ALPPL2; ALPPL; Alkaline phosphatase, placental-likeALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like; PLAP-like |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 60kD |
| Cellular localization | Cell membrane; Lipid-anchor, GPI-anchor . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Thiamine metabolism;>>Folate biosynthesis;>>Metabolic pathways;>>Biosynthesis of cofactors |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2. AA range:1-50 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
PLAP
Also known as
ALPP; PLAP; Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; ALPPL2; ALPPL; Alkaline phosphatase, placental-likeALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like; PLAP-like
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2. AA range:1-50
Observed band
60kD
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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