ALDH2 rabbit pAb from ELK Biotechnology Read more ›
ALDH2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ALDH2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform,
| Specificity | ALDH2 Polyclonal Antibody detects endogenous levels of ALDH2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of A549 cells using ALDH2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4085 |
| Target | ALDH2 |
| Research areas | >>Glycolysis / Gluconeogenesis, >>Ascorbate and aldarate metabolism, >>Fatty acid degradation, >>Valine, leucine and isoleucine degradation, >>Lysine degradation, >>Arginine and proline metabolism, >>Histidine metabolism, >>Tryptophan metabolism, >>beta-Alanine metabolism, >>Glycerolipid metabolism, >>Pyruvate metabolism, >>Pantothenate and CoA biosynthesis, >>Metabolic pathways, >>Biosynthesis of cofactors, >>Alcoholic liver disease |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human ALDH2. AA range:41-90 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Adipocyte,Brain,Cajal-Retzius cell,Liver,Lymph,Muscle,Small |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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