Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97989

HSP 90 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

HSP 90 rabbit pAb from ELK Biotechnology Read more ›

Placeholder image
Zoom
Placeholder image

Product Description

HSP 90 rabbit pAb is a research-use antibody from ELK Biotechnology for studying HSP 90 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

Additional antibody information

Specificity HSP 90 Polyclonal Antibody detects endogenous levels of HSP 90 protein.

Key Facts

Product typePrimary Antibody Target: HSP 90
Collaborator ELK Biotechnology
Partner model ES4057
Target HSP 90
Research areas>>Protein processing in endoplasmic reticulum, >>PI3K-Akt signaling pathway, >>Necroptosis, >>Antigen processing and presentation, >>NOD-like receptor signaling pathway, >>IL-17 signaling pathway, >>Th17 cell differentiation, >>Progesterone-mediated oocyte maturation, >>Estrogen signaling pathway, >>Salmonella infection, >>Pathways in cancer, >>Chemical carcinogenesis - receptor activation, >>Prostate cancer, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human HSP90A/B around the non-acetylation site of Lys292/284. AA range:251-300
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Adult brain,Brain,Brain cortex,Breast,Cajal-Retzius
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
HSP 90
Also known as
HSP90AA1; HSP90A; HSPC1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Renal carcinoma antigen NY-REN-38; HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human HSP90A/B around the non-acetylation site of Lys292/284. AA range:251-300
Observed band
85kD
Cellular localization
Nucleus . Cytoplasm . Melanosome . Cell membrane . Mitochondrion . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.