Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97985

NUMB rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NUMB rabbit pAb from ELK Biotechnology Read more ›

Placeholder image
Zoom
Placeholder image

Product Description

NUMB rabbit pAb is a research-use antibody from ELK Biotechnology for studying NUMB in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

Additional antibody information

Specificity NUMB Polyclonal Antibody detects endogenous levels of NUMB protein.

Key Facts

Product typePrimary Antibody Target: NUMB
Collaborator ELK Biotechnology
Partner model ES4050
Target NUMB
Research areas>>Notch signaling pathway
Reactivity Human,Mouse,Rat
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000; IF/ICC 1:100-500;ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from the Internal region of human NUMB.
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system B cells,Blood,Brain,Cervix carcinoma,Epithelium,Fetal brain,HeLa cells,Live
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000; IF/ICC 1:100-500;ELISA 1:5000-20000
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
NUMB
Also known as
NUMB; Protein numb homolog; h-Numb; Protein S171
Concentration
1 mg/ml
Working concentration
WB 1:500-2000; IF/ICC 1:100-500;ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIFELISA
Immunogen
Synthesized peptide derived from the Internal region of human NUMB.
Observed band
70kD
Cellular localization
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Localizes to perinuclear endosomes in an AAK1-dependent manner. .
Dilution range
WB 1:500-2000; IF/ICC 1:100-500;ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.