SCCA1/2 rabbit pAb from ELK Biotechnology Read more ›
SCCA1/2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SCCA1/2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
developmental stage:Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.,function:May act as a protease inhibitor to modulate the host immune response against tumor cells.,similarity:Belongs to the serpin family.,similarity:Belongs to the serpin family. Ov-serpin subfamily.,subcellular location:Seems to also be secreted in plasma by cancerous cells but at a low level.,tissue specificity:Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).,
| Specificity | SCCA1/2 Polyclonal Antibody detects endogenous levels of SCCA1/2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat, 293 cells using SCCA1/2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4040 |
| Target | SCCA1/2 |
| Research areas | >>Amoebiasis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SERPINB3/4. AA range:111-160 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Squamous cells. Expressed in some hepatocellular carcinoma (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
