ABM97972
IFN-ε rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
IFN-ε rabbit pAb from ELK Biotechnology Read more ›
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Product Description
IFN-ε rabbit pAb is an ELK Biotechnology antibody for research workflows involving IFN-ε.
Background
similarity:Belongs to the alpha/beta interferon family.,tissue specificity:Expressed in prostate cancer cell line PC-3, amnion-derived WISH cells, SK-MEL28 melanoma cells and Daudi cells and very weakly in MCF-7 human breast cancer cells. Not detected in Namalwa cells (Burkitt's lymphoma) or in Jurkat (T) cells.,
Additional antibody information
| Specificity | IFN-ε Polyclonal Antibody detects endogenous levels of IFN-ε protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat cells using IFN-ε Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from Jurkat cells, using IFNE Antibody. |
Key Facts
Product type
Primary Antibody›
Target: IFN-ε
| Target | IFN-ε |
| Also known as | IFNE; IFNE1; Interferon epsilon; IFN-epsilon; Interferon epsilon-1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 24kD |
| Cellular localization | Secreted . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cytokine-cytokine receptor interaction;>>RIG-I-like receptor signaling pathway;>>JAK-STAT signaling pathway |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human IFNE. AA range:159-208 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
IFN-ε
Also known as
IFNE; IFNE1; Interferon epsilon; IFN-epsilon; Interferon epsilon-1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human IFNE. AA range:159-208
Observed band
24kD
Cellular localization
Secreted .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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