Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97972

IFN-ε rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IFN-ε rabbit pAb from ELK Biotechnology Read more ›

IFN-ε rabbit pAb
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IFN-ε rabbit pAb

Product Description

IFN-ε rabbit pAb is a research-use antibody from ELK Biotechnology for studying IFN-ε in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

similarity:Belongs to the alpha/beta interferon family.,tissue specificity:Expressed in prostate cancer cell line PC-3, amnion-derived WISH cells, SK-MEL28 melanoma cells and Daudi cells and very weakly in MCF-7 human breast cancer cells. Not detected in Namalwa cells (Burkitt's lymphoma) or in Jurkat (T) cells.,

Additional antibody information

Specificity IFN-ε Polyclonal Antibody detects endogenous levels of IFN-ε protein.
Validation evidence captions Western Blot analysis of Jurkat cells using IFN-ε Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from Jurkat cells, using IFNE Antibody.

Key Facts

Product typePrimary Antibody Target: IFN-ε
Collaborator ELK Biotechnology
Partner model ES4034
Target IFN-ε
Research areas>>Cytokine-cytokine receptor interaction, >>RIG-I-like receptor signaling pathway, >>JAK-STAT signaling pathway
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the C-terminal region of human IFNE. AA range:159-208
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Endometrium-specific.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
IFN-ε
Also known as
IFNE; IFNE1; Interferon epsilon; IFN-epsilon; Interferon epsilon-1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human IFNE. AA range:159-208
Observed band
24kD
Cellular localization
Secreted .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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