ABM97968
PDGF-D rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
PDGF-D rabbit pAb from ELK Biotechnology Read more ›
Related formats
Zoom
Product Description
PDGF-D rabbit pAb is an ELK Biotechnology antibody for research workflows involving PDGF-D.
Background
platelet derived growth factor D(PDGFD) Homo sapiens The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | PDGF-D Polyclonal Antibody detects endogenous levels of PDGF-D protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of L929 cells using PDGF-D Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100 | Western blot analysis of lysate from L929 cells, using PDGFD Antibody. |
Key Facts
Product type
Primary Antibody›
Target: PDGF-D
| Target | PDGF-D |
| Also known as | PDGFD; IEGF; SCDGFB; MSTP036; Platelet-derived growth factor D; PDGF-D; Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 42kD |
| Cellular localization | Secreted . Released by platelets upon wounding. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>Phospholipase D signaling pathway;>>PI3K-Akt signaling pathway;>>Focal adhesion;>>Gap junction;>>Regulation of actin cytoskeleton;>>Prostate cancer;>>Melanoma;>>Choline metabolism in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human PDGFD. AA range:311-360 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Why researchers trust ABMIUM
2,000+
Researchers trust us
50,000+
Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
PDGF-D
Also known as
PDGFD; IEGF; SCDGFB; MSTP036; Platelet-derived growth factor D; PDGF-D; Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human PDGFD. AA range:311-360
Observed band
42kD
Cellular localization
Secreted . Released by platelets upon wounding.
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
Customer Reviews
Recently Viewed