ABM97943
CD300g rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
CD300g rabbit pAb from ELK Biotechnology Read more ›
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Product Description
CD300g rabbit pAb is an ELK Biotechnology antibody for research workflows involving CD300g.
Background
Members of the CD300 (see MIM 606786)-like (CD300L) family, such as CD300LG, are widely expressed on hematopoietic cells. All CD300L proteins are type I cell surface glycoproteins that contain a single immunoglobulin (Ig) V-like domain (Takatsu et al., 2006 [PubMed 16876123]).[supplied by OMIM, Mar 2008],
Additional antibody information
| Specificity | CD300g Polyclonal Antibody detects endogenous levels of CD300g protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of L929 cells using CD300g Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-prostate-cancer, antibody was diluted at 1:100 | Western blot analysis of lysate from L929 cells, using CD300LG Antibody. |
Key Facts
Product type
Primary Antibody›
Target: CD300g
| Target | CD300g |
| Also known as | CD300LG; CLM9; TREM4; CMRF35-like molecule 9; CLM-9; CD300 antigen-like family member G; Triggering receptor expressed on myeloid cells 4; TREM-4; CD300g |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 36kD |
| Cellular localization | Apical cell membrane ; Single-pass type I membrane protein . Basolateral cell membrane ; Single-pass type I membrane protein . Endosome, multivesicular body membrane ; Single-pass type I membrane protein . Transcytoses across the cytoplasm. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CD300LG. AA range:41-90 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
CD300g
Also known as
CD300LG; CLM9; TREM4; CMRF35-like molecule 9; CLM-9; CD300 antigen-like family member G; Triggering receptor expressed on myeloid cells 4; TREM-4; CD300g
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD300LG. AA range:41-90
Observed band
36kD
Cellular localization
Apical cell membrane ; Single-pass type I membrane protein . Basolateral cell membrane ; Single-pass type I membrane protein . Endosome, multivesicular body membrane ; Single-pass type I membrane protein . Transcytoses across the cytoplasm. .
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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