IL-1RI rabbit pAb from ELK Biotechnology Read more ›
IL-1RI rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL-1RI in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013],
| Specificity | IL-1RI Polyclonal Antibody detects endogenous levels of IL-1RI protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of MCF7 cells using IL-1RI Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES3993 |
| Target | IL-1RI |
| Research areas | >>MAPK signaling pathway, >>Cytokine-cytokine receptor interaction, >>NF-kappa B signaling pathway, >>Osteoclast differentiation, >>Hematopoietic cell lineage, >>Th17 cell differentiation, >>Inflammatory mediator regulation of TRP channels, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Amoebiasis, >>Human cytomegalovirus infection, >>Human T-cell leukemia virus 1 infection, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human IL1R1. AA range:31-80 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in T-helper cell subsets. Preferentially expressed in T-helper 1 (Th1) cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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