ABM97936
CD116 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
CD116 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
CD116 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CD116.
Background
The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | CD116 Polyclonal Antibody detects endogenous levels of CD116 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of L929 cells using CD116 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100 | Western blot analysis of lysate from L929 cells, using CSF2RA Antibody. |
Key Facts
Product type
Primary Antibody›
Target: CD116
| Target | CD116 |
| Also known as | CSF2RA; CSF2R; CSF2RY; Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CDw116; CD116 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 44kD |
| Cellular localization | Cell membrane; Single-pass type I membrane protein.; Isoform 3: Secreted .; Isoform 4: Secreted .; Isoform 6: Secreted . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cytokine-cytokine receptor interaction;>>JAK-STAT signaling pathway;>>Hematopoietic cell lineage;>>Pathways in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CSF2RA. AA range:121-170 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
CD116
Also known as
CSF2RA; CSF2R; CSF2RY; Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CDw116; CD116
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CSF2RA. AA range:121-170
Observed band
44kD
Cellular localization
Cell membrane; Single-pass type I membrane protein.; Isoform 3: Secreted .; Isoform 4: Secreted .; Isoform 6: Secreted .
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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