Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97934

CD107a rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CD107a rabbit pAb from ELK Biotechnology Read more ›

CD107a rabbit pAb
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CD107a rabbit pAb

Product Description

CD107a rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD107a in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CD107a Polyclonal Antibody detects endogenous levels of CD107a protein.
Validation evidence captions Western Blot analysis of L929 cells using CD107a Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from L929 cells, using LAMP1 Antibody.

Key Facts

Product typePrimary Antibody Target: CD107a
Collaborator ELK Biotechnology
Partner model ES3990
Target CD107a
Research areas>>Autophagy - animal, >>Lysosome, >>Phagosome, >>Tuberculosis
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human LAMP1. AA range:211-260
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Liver,Pancreas,Placenta,Plasma,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CD107a
Also known as
LAMP1; Lysosome-associated membrane glycoprotein 1; LAMP-1; Lysosome-associated membrane protein 1; CD107 antigen-like family member A; CD107a
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human LAMP1. AA range:211-260
Observed band
120kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Endosome membrane; Single-pass type I membrane protein . Lysosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Cytolytic granule membrane ; Single-pass type I membrane protein . This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity). Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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