Primary Antibody ISO 9001|ABMIUM Verified™
ABM97925

CD55 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CD55 rabbit pAb from ELK Biotechnology Read more ›

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CD55 rabbit pAb
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CD55 rabbit pAb

Product Description

CD55 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CD55.

Background

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014],

Additional antibody information

Specificity CD55 Polyclonal Antibody detects endogenous levels of CD55 protein.
Validation evidence captions Western Blot analysis of K562 cells using CD55 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from K562 cells, using CD55 Antibody.

Key Facts

Product type Primary Antibody Target: CD55
Target CD55
Also known as CD55; CR; DAF; Complement decay-accelerating factor; CD55
Concentration 1 mg/ml
Working concentration Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Species reactivity Human;Rat;Mouse;
Observed band 42kD
Cellular localization Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform 3: Secreted .; Isoform 4: Secreted .; Isoform 5: Secreted .; Isoform 6: Cell membrane ; Lipid-anchor, GPI-anchor .; Isoform 7: Cell membrane ; Lipid-anchor, GPI-anchor .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area >>Complement and coagulation cascades;>>Hematopoietic cell lineage;>>Viral myocarditis
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBELISA
Dilution range Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human CD55. AA range:241-290
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CD55
Also known as
CD55; CR; DAF; Complement decay-accelerating factor; CD55
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD55. AA range:241-290
Observed band
42kD
Cellular localization
Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform 3: Secreted .; Isoform 4: Secreted .; Isoform 5: Secreted .; Isoform 6: Cell membrane ; Lipid-anchor, GPI-anchor .; Isoform 7: Cell membrane ; Lipid-anchor, GPI-anchor .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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