CD55 rabbit pAb
CD55 rabbit pAb from ELK Biotechnology Read more ›

Product Description
CD55 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CD55.
Background
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014],
Additional antibody information
| Specificity | CD55 Polyclonal Antibody detects endogenous levels of CD55 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of K562 cells using CD55 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from K562 cells, using CD55 Antibody. |
Key Facts
| Target | CD55 |
| Also known as | CD55; CR; DAF; Complement decay-accelerating factor; CD55 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 42kD |
| Cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform 3: Secreted .; Isoform 4: Secreted .; Isoform 5: Secreted .; Isoform 6: Cell membrane ; Lipid-anchor, GPI-anchor .; Isoform 7: Cell membrane ; Lipid-anchor, GPI-anchor . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Complement and coagulation cascades;>>Hematopoietic cell lineage;>>Viral myocarditis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CD55. AA range:241-290 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.