ABM97914
PEPCK-C rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
PEPCK-C rabbit pAb from ELK Biotechnology Read more ›
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Product Description
PEPCK-C rabbit pAb is an ELK Biotechnology antibody for research workflows involving PEPCK-C.
Background
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | PEPCK-C Polyclonal Antibody detects endogenous levels of PEPCK-C protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HeLa, MCF7 cells using PEPCK-C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-liver, antibody was diluted at 1:100 |
Key Facts
Product type
Primary Antibody›
Target: PEPCK-C
| Target | PEPCK-C |
| Also known as | PCK1; PEPCK1; Phosphoenolpyruvate carboxykinase, cytosolic GTP; PEPCK-C; Phosphoenolpyruvate carboxylase |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 65kD |
| Cellular localization | Cytoplasm, cytosol . Endoplasmic reticulum . Phosphorylation at Ser-90 promotes translocation to the endoplasmic reticulum. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Glycolysis / Gluconeogenesis;>>Citrate cycle (TCA cycle);>>Pyruvate metabolism;>>Metabolic pathways;>>PPAR signaling pathway;>>FoxO signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Proximal tubule bicarbonate reclamation |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human PCK1. AA range:491-540 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
PEPCK-C
Also known as
PCK1; PEPCK1; Phosphoenolpyruvate carboxykinase, cytosolic GTP; PEPCK-C; Phosphoenolpyruvate carboxylase
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human PCK1. AA range:491-540
Observed band
65kD
Cellular localization
Cytoplasm, cytosol . Endoplasmic reticulum . Phosphorylation at Ser-90 promotes translocation to the endoplasmic reticulum. .
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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