PEPCK-C rabbit pAb from ELK Biotechnology Read more ›
PEPCK-C rabbit pAb is a research-use antibody from ELK Biotechnology for studying PEPCK-C in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008],
| Specificity | PEPCK-C Polyclonal Antibody detects endogenous levels of PEPCK-C protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HeLa, MCF7 cells using PEPCK-C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-liver, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES3966 |
| Target | PEPCK-C |
| Research areas | >>Glycolysis / Gluconeogenesis, >>Citrate cycle (TCA cycle), >>Pyruvate metabolism, >>Metabolic pathways, >>PPAR signaling pathway, >>FoxO signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Insulin signaling pathway, >>Adipocytokine signaling pathway, >>Glucagon signaling pathway, >>Insulin resistance, >>Proximal tubule bicarbonate reclamation |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human PCK1. AA range:491-540 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Major sites of expression are liver, kidney and adipocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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