DPYD rabbit pAb from ELK Biotechnology Read more ›
DPYD rabbit pAb is a research-use antibody from ELK Biotechnology for studying DPYD in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
| Specificity | DPYD Polyclonal Antibody detects endogenous levels of DPYD protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HepG2 cells using DPYD Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES3957 |
| Target | DPYD |
| Research areas | >>Pyrimidine metabolism, >>beta-Alanine metabolism, >>Pantothenate and CoA biosynthesis, >>Drug metabolism - other enzymes, >>Metabolic pathways |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human DPYD. AA range:351-400 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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