Kallistatin rabbit pAb
Kallistatin rabbit pAb from ELK Biotechnology Read more ›
Product Description
Kallistatin rabbit pAb is an ELK Biotechnology antibody for research workflows involving Kallistatin.
Background
function:Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.,miscellaneous:Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.,PTM:The N-terminus is blocked.,similarity:Belongs to the serpin family.,subunit:Monomer and some homodimers.,tissue specificity:Expressed by the liver and secreted in plasma.,
Additional antibody information
| Specificity | Kallistatin Polyclonal Antibody detects endogenous levels of Kallistatin protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of K562 cells using Kallistatin Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100 | Western blot analysis of lysate from K562 cells, using SERPINA4 Antibody. |
Key Facts
| Target | Kallistatin |
| Also known as | SERPINA4; KST; PI4; Kallistatin; Kallikrein inhibitor; Peptidase inhibitor 4; PI-4; Serpin A4 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 47kD |
| Cellular localization | Secreted. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SERPINA4. AA range:161-210 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.