ABM97884
SP-A rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
SP-A rabbit pAb from ELK Biotechnology Read more ›
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Product Description
SP-A rabbit pAb is an ELK Biotechnology antibody for research workflows involving SP-A.
Background
This gene is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication. [provided by RefSeq, Sep 2009],
Additional antibody information
| Specificity | SP-A Polyclonal Antibody detects endogenous levels of SP-A protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 22RV-1 and SW480 cell lysisi, mouse lung cells using SP-A Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100 | Western blot analysis of lysate from K562 cells, using SFTPA1/2 Antibody. |
Key Facts
Product type
Primary Antibody›
Target: SP-A
| Target | SP-A |
| Also known as | SFTPA2; COLEC5; PSAP; SFTP1; SFTPA; SFTPA2B; Pulmonary surfactant-associated protein A2; PSP-A; PSPA; SP-A; SP-A2; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-5; SFTPA1; COLEC4; PSAP;SFTP1; SFTPA; SFTPA1B; Pulmonary surfactant-associated protein A1; PSP-A; PSPA; SP-A; SP-A1; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-4 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 34kD |
| Cellular localization | Secreted . Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Phagosome;>>Pertussis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SFTPA1/2. AA range:141-190 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
SP-A
Also known as
SFTPA2; COLEC5; PSAP; SFTP1; SFTPA; SFTPA2B; Pulmonary surfactant-associated protein A2; PSP-A; PSPA; SP-A; SP-A2; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-5; SFTPA1; COLEC4; PSAP;SFTP1; SFTPA; SFTPA1B; Pulmonary surfactant-associated protein A1; PSP-A; PSPA; SP-A; SP-A1; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-4
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human SFTPA1/2. AA range:141-190
Observed band
34kD
Cellular localization
Secreted . Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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