ABM97867
Amylase rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Amylase rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Amylase rabbit pAb is an ELK Biotechnology antibody for research workflows involving Amylase.
Background
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Amylase Polyclonal Antibody detects endogenous levels of Amylase protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HeLa cells using Amylase Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-liver, antibody was diluted at 1:100 |
Key Facts
Product type
Primary Antibody›
Target: Amylase
| Target | Amylase |
| Also known as | AMY1A; AMY1; AMY1B; AMY1; AMY1C; AMY1; Alpha-amylase 1; 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY2A; Pancreatic alpha-amylase; PA;1,4-alpha-D-glucan glucanohydrolase; AMY2B; Alpha-amylase 2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 60kD |
| Cellular localization | extracellular space,extracellular exosome, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human AMY1/2. AA range:61-110 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Amylase
Also known as
AMY1A; AMY1; AMY1B; AMY1; AMY1C; AMY1; Alpha-amylase 1; 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY2A; Pancreatic alpha-amylase; PA;1,4-alpha-D-glucan glucanohydrolase; AMY2B; Alpha-amylase 2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human AMY1/2. AA range:61-110
Observed band
60kD
Cellular localization
extracellular space,extracellular exosome,
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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