Actin-α cardiac muscle rabbit pAb
Actin-α cardiac muscle rabbit pAb from ELK Biotechnology Read more ›
Product Description
Actin-α cardiac muscle rabbit pAb is an ELK Biotechnology antibody for research workflows involving Actin-α cardiac muscle.
Background
Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Actin-α cardiac muscle Polyclonal Antibody detects endogenous levels of Actin-α cardiac muscle protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of mouse heart, mouse lung cells using Actin-α cardiac muscle Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100 |
Key Facts
| Target | Actin-α cardiac muscle |
| Also known as | ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 42kD |
| Cellular localization | Cytoplasm, cytoskeleton. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cardiac muscle contraction;>>Adrenergic signaling in cardiomyocytes;>>Hypertrophic cardiomyopathy;>>Dilated cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from Actin-α cardiac muscle . at AA range: 1-80 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.