Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97833

ATP1AL1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ATP1AL1 rabbit pAb from ELK Biotechnology Read more ›

ATP1AL1 rabbit pAb
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ATP1AL1 rabbit pAb

Product Description

ATP1AL1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATP1AL1 in WB, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],

Additional antibody information

Specificity ATP1AL1 Polyclonal Antibody detects endogenous levels of ATP1AL1 protein.
Validation evidence captions Western Blot analysis of extracts from rat stomach, using ATP1AL1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: ATP1AL1
Collaborator ELK Biotechnology
Partner model ES3829
Target ATP1AL1
Research areas>>Oxidative phosphorylation, >>Metabolic pathways
Reactivity Human,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from ATP1AL1 . at AA range: 380-460
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in airway epithelial cells (at protein level) (PubMed:29391451). Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ATP1AL1
Also known as
ATP12A; ATP1AL1; Potassium-transporting ATPase alpha chain 2; Non-gastric H(+)/K(+) ATPase subunit alpha; Proton pump
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from ATP1AL1 . at AA range: 380-460
Observed band
115kD
Cellular localization
Apical cell membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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