Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97803

AR-β2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

AR-β2 rabbit pAb from ELK Biotechnology Read more ›

AR-β2 rabbit pAb
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AR-β2 rabbit pAb

Product Description

AR-β2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Adrenergic Receptor β2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity AR-β2 Polyclonal Antibody detects endogenous levels of AR-β2 protein.
Validation evidence captions Western Blot analysis of extracts from K562 cells, using AR-β2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: Adrenergic Receptor β2
Collaborator ELK Biotechnology
Partner model ES3778
Target Adrenergic Receptor β2
Research areas>>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Adrenergic signaling in cardiomyocytes, >>Regulation of lipolysis in adipocytes, >>Renin secretion, >>Salivary secretion, >>Chemical carcinogenesis - receptor activation
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from AR-β2 . at AA range: 300-380
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Adrenergic Receptor β2
Also known as
ADRB2; ADRB2R; B2AR; Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from AR-β2 . at AA range: 300-380
Observed band
46kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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