EEA1 rabbit pAb from ELK Biotechnology Read more ›
EEA1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying EEA1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
disease:Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.,domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.,function:Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 1 FYVE-type zinc finger.,subunit:Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.,
| Specificity | EEA1 Polyclonal Antibody detects endogenous levels of EEA1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of extracts from K562 cells, using EEA1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from A431 cells , using EEA1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3758 |
| Target | EEA1 |
| Research areas | >>Endocytosis, >>Phagosome, >>Tuberculosis |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EEA1. AA range:205-254 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Aorta,Cervix carcinoma,Fetal brain cortex,Skeletal muscle, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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