Primary Antibody ISO 9001|ABMIUM Verified™
ABM97784

CHD1L rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CHD1L rabbit pAb from ELK Biotechnology Read more ›

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CHD1L rabbit pAb
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CHD1L rabbit pAb

Product Description

CHD1L rabbit pAb is an ELK Biotechnology antibody for research workflows involving CHD1L.

Background

chromodomain helicase DNA binding protein 1 like(CHD1L) Homo sapiens This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Several alternatively spliced transcripts variants have been described for this gene. [provided by RefSeq, Jan 2012],

Additional antibody information

Specificity CHD1L Polyclonal Antibody detects endogenous levels of CHD1L protein.
Validation evidence captions Western Blot analysis of extracts from NIH-3T3, Jurkat, A549 cells, using CHD1L Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of lysates from HepG2 cells, using CHD1L antibody.

Key Facts

Product type Primary Antibody Target: CHD1L
Target CHD1L
Also known as CHD1L; ALC1; Chromodomain-helicase-DNA-binding protein 1-like; Amplified in liver cancer protein 1
Concentration 1 mg/ml
Working concentration Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Species reactivity Human;Mouse
Observed band 101kD
Cellular localization Nucleus . Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1. .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBELISA
Dilution range Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen The antiserum was produced against synthesized peptide derived from human CHD1L. AA range:545-594
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CHD1L
Also known as
CHD1L; ALC1; Chromodomain-helicase-DNA-binding protein 1-like; Amplified in liver cancer protein 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CHD1L. AA range:545-594
Observed band
101kD
Cellular localization
Nucleus . Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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