Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97782

ALDH3A1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ALDH3A1 rabbit pAb from ELK Biotechnology Read more ›

ALDH3A1 rabbit pAb
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ALDH3A1 rabbit pAb

Product Description

ALDH3A1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ALDH3A1 in WB, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008],

Additional antibody information

Specificity ALDH3A1 Polyclonal Antibody detects endogenous levels of ALDH3A1 protein.
Validation evidence captions Western Blot analysis of extracts from A549, K562 cells, using ALDH3A1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from A549 cells, using ALDH3A1 antibody.

Key Facts

Product typePrimary Antibody Target: ALDH3A1
Collaborator ELK Biotechnology
Partner model ES3755
Target ALDH3A1
Research areas>>Glycolysis / Gluconeogenesis, >>Histidine metabolism, >>Tyrosine metabolism, >>Phenylalanine metabolism, >>beta-Alanine metabolism, >>Metabolism of xenobiotics by cytochrome P450, >>Drug metabolism - cytochrome P450, >>Metabolic pathways
Reactivity Human,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ALDH3A1. AA range:236-285
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system High levels in stomach, esophagus and lung; low level in the liver and kidney.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ALDH3A1
Also known as
ALDH3A1; ALDH3; Aldehyde dehydrogenase, dimeric NADP-preferring; ALDHIII; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase family 3 member A1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ALDH3A1. AA range:236-285
Observed band
50kD
Cellular localization
Cytoplasm .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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