Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97767

ZFP106 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ZFP106 rabbit pAb from ELK Biotechnology Read more ›

ZFP106 rabbit pAb
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ZFP106 rabbit pAb

Product Description

ZFP106 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ZFP106 in WB, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 2 C2H2-type zinc fingers.,similarity:Contains 6 WD repeats.,subunit:Interacts with TSG118.,

Additional antibody information

Specificity ZFP106 Polyclonal Antibody detects endogenous levels of ZFP106 protein.
Validation evidence captions Western Blot analysis of various cells using ZFP106 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of COS7 cells using ZFP106 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from COS7 cells, using ZFP106 antibody.

Key Facts

Product typePrimary Antibody Target: ZFP106
Collaborator ELK Biotechnology
Partner model ES3728
Target ZFP106
Research areasAntibody Research
Reactivity Human,Mouse,Monkey
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ZFP106. AA range:1833-1882
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Epithelium,Human skeletal muscle,Prostate,Skeletal muscle,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
Monkey
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ZFP106
Also known as
ZFP106; SH3BP3; ZNF474; Zinc finger protein 106 homolog; Zfp-106; Zinc finger protein 474
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ZFP106. AA range:1833-1882
Observed band
209kD
Cellular localization
Nucleus, nucleolus . Nucleus speckle . Colocalizes with RBM39 in nuclear speckles. Inhibition of RNA synthesis, or overexpression of KNOP1, induces translocation from nuclear speckles to the nucleolus. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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