ABM97738
TSSK 4 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
TSSK 4 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
TSSK 4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving TSSK 4.
Background
This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
Additional antibody information
| Specificity | TSSK 4 Polyclonal Antibody detects endogenous levels of TSSK 4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using TSSK 4 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from HT-29 cells, using TSSK4 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using TSSK4 antibody. |
Key Facts
Product type
Primary Antibody›
Target: TSSK 4
| Target | TSSK 4 |
| Also known as | TSSK4; C14orf20; STK22E; TSSK5; Testis-specific serine/threonine-protein kinase 4; TSK-4; TSSK-4; Testis-specific kinase 4; Serine/threonine-protein kinase 22E |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 40kD |
| Cellular localization | Cytoplasmic vesicle, secretory vesicle, acrosome . Cell projection, cilium, flagellum . In spermatozoa, present in the sperm head and in the flagellum. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TSSK4. AA range:211-260 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
TSSK 4
Also known as
TSSK4; C14orf20; STK22E; TSSK5; Testis-specific serine/threonine-protein kinase 4; TSK-4; TSSK-4; Testis-specific kinase 4; Serine/threonine-protein kinase 22E
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TSSK4. AA range:211-260
Observed band
40kD
Cellular localization
Cytoplasmic vesicle, secretory vesicle, acrosome . Cell projection, cilium, flagellum . In spermatozoa, present in the sperm head and in the flagellum. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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