Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97738

TSSK 4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TSSK 4 rabbit pAb from ELK Biotechnology Read more ›

TSSK 4 rabbit pAb
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TSSK 4 rabbit pAb

Product Description

TSSK 4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TSSK 4 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],

Additional antibody information

Specificity TSSK 4 Polyclonal Antibody detects endogenous levels of TSSK 4 protein.
Validation evidence captions Western Blot analysis of various cells using TSSK 4 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from HT-29 cells, using TSSK4 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using TSSK4 antibody.

Key Facts

Product typePrimary Antibody Target: TSSK 4
Collaborator ELK Biotechnology
Partner model ES3651
Target TSSK 4
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human TSSK4. AA range:211-260
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed only in the testis.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TSSK 4
Also known as
TSSK4; C14orf20; STK22E; TSSK5; Testis-specific serine/threonine-protein kinase 4; TSK-4; TSSK-4; Testis-specific kinase 4; Serine/threonine-protein kinase 22E
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TSSK4. AA range:211-260
Observed band
40kD
Cellular localization
Cytoplasmic vesicle, secretory vesicle, acrosome . Cell projection, cilium, flagellum . In spermatozoa, present in the sperm head and in the flagellum. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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