Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97732

TRAF3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TRAF3 rabbit pAb from ELK Biotechnology Read more ›

TRAF3 rabbit pAb
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TRAF3 rabbit pAb

Product Description

TRAF3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRAF3 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

TNF receptor associated factor 3(TRAF3) Homo sapiens The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq, Dec 2010],

Additional antibody information

Specificity TRAF3 Polyclonal Antibody detects endogenous levels of TRAF3 protein.
Validation evidence captions Western Blot analysis of various cells using TRAF3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of COLO205 cells using TRAF3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from HeLa cells, using TRAF3 antibody.

Key Facts

Product typePrimary Antibody Target: TRAF3
Collaborator ELK Biotechnology
Partner model ES3635
Target TRAF3
Research areas>>NF-kappa B signaling pathway, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>IL-17 signaling pathway, >>TNF signaling pathway, >>Alcoholic liver disease, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Viral carcinogenesis, >>Small cell lung cancer, >>Lipid and atherosclerosis
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human TRAF3. AA range:240-289
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Fetal brain,Lymphoma,T-cell,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TRAF3
Also known as
TRAF3; CAP1; CRAF1; TNF receptor-associated factor 3; CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TRAF3. AA range:240-289
Observed band
64kD
Cellular localization
Cytoplasm . Endosome . Mitochondrion . Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Co-localized to mitochondria with TRIM35 (PubMed:32562145). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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