Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97715

TG1019 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TG1019 rabbit pAb from ELK Biotechnology Read more ›

TG1019 rabbit pAb
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TG1019 rabbit pAb

Product Description

TG1019 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TG1019 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Receptor for eicosanoids and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE) and arachidonic acid. Seems to be coupled to the G(i)/G(o), families of heteromeric G proteins.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expressed in various tissues except brain. Expression is more intense in liver, kidney, peripheral leukocyte, lung, and spleen than in other tissues. Highly expressed in eosinophils, neutrophils, and lung macrophages.,

Additional antibody information

Specificity TG1019 Polyclonal Antibody detects endogenous levels of TG1019 protein.
Validation evidence captions Western Blot analysis of various cells using TG1019 Polyclonal Antibody | Immunofluorescence analysis of MCF7 cells, using OXER1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from MCF-7 and Jurkat cells, using OXER1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HUVECcells using OXER1 antibody.

Key Facts

Product typePrimary Antibody Target: TG1019
Collaborator ELK Biotechnology
Partner model ES3591
Target TG1019
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human OXER1. AA range:212-261
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in various tissues except brain. Expression is more intense in liver, kidney, peripheral leukocyte, lung, and spleen than in other tissues. Highly expressed in eosinophils, neutrophils, and lung macrophages.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TG1019
Also known as
OXER1; GPR170; TG1019; Oxoeicosanoid receptor 1; 5-oxo-ETE G-protein coupled receptor; G-protein coupled receptor 170; G-protein coupled receptor R527; G-protein coupled receptor TG1019
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human OXER1. AA range:212-261
Observed band
46kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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