ABM97713
TFDP1 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
TFDP1 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
TFDP1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving TFDP1.
Background
This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.[provided by RefSeq, Jan 2009],
Additional antibody information
| Specificity | TFDP1 Polyclonal Antibody detects endogenous levels of TFDP1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using TFDP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Immunofluorescence analysis of HeLa cells, using DP-1 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DP-1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa cells, using DP-1 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: TFDP1
| Target | TFDP1 |
| Also known as | TFDP1; DP1; Transcription factor Dp-1; DRTF1-polypeptide 1; DRTF1; E2F dimerization partner 1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 55kD |
| Cellular localization | Nucleus . Cytoplasm . Shuttles between the cytoplasm and nucleus and translocates into the nuclear compartment upon heterodimerization with E2F1. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell cycle;>>TGF-beta signaling pathway |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DP-1. AA range:361-410 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
TFDP1
Also known as
TFDP1; DP1; Transcription factor Dp-1; DRTF1-polypeptide 1; DRTF1; E2F dimerization partner 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human DP-1. AA range:361-410
Observed band
55kD
Cellular localization
Nucleus . Cytoplasm . Shuttles between the cytoplasm and nucleus and translocates into the nuclear compartment upon heterodimerization with E2F1. .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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