STEAP1 rabbit pAb from ELK Biotechnology Read more ›
STEAP1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying STEAP1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008],
| Specificity | STEAP1 Polyclonal Antibody detects endogenous levels of STEAP1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using STEAP1 Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunohistochemistry analysis of STEAP1 antibody in paraffin-embedded human prostate carcinoma tissue. | Western blot analysis of lysate from A549, using STEAP1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3521 |
| Target | STEAP1 |
| Research areas | >>Mineral absorption |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STEAP1. AA range:31-80 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed. Highly expressed in prostate tumors. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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