SSBP1 rabbit pAb from ELK Biotechnology Read more ›
SSBP1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SSBP1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Cow. Specifications and supporting documents below should be reviewed when planning the experiment.
SSBP1 is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al., 1995 [PubMed 7789991]). It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010],
| Specificity | SSBP1 Polyclonal Antibody detects endogenous levels of SSBP1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using SSBP1 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using MtSSB Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using MtSSB Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HUVEC and HeLa cells, using MtSSB Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3502 |
| Target | SSBP1 |
| Research areas | >>DNA replication, >>Mismatch repair, >>Homologous recombination |
| Reactivity | Human,Mouse,Rat,Cow |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MtSSB. AA range:99-148 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in retinal ganglion cells, photoreceptors, pigmented epithelium and fibroblasts (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Cow |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Cow | — | — |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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